Recombinant Human FCGR1A/CD64 Protein (His tag)

Recombinant Human FCGR1A/CD64 Protein (His tag)

SKU #	PDMH100112
Expression Host	HEK293 Cells

Description

Synonyms	CD antigen CD64,Fc-gamma RI,Fc-gamma RIA,FcRI,FcgammaRIa,High affinity immunoglobulin gamma Fc receptor I,IgG Fc receptor I
Species	Human
Expression Host	HEK293 Cells
Sequence	Met1-Pro288
Accession	P12314
Calculated Molecular Weight	31.6 kDa
Observed Molecular Weight	35 kDa
Tag	C-His
Bio-activity	Not validated for activity

Properties

Purity	> 95% as determined by reducing SDS-PAGE.
Endotoxin	Please contact us for more information.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Background

CD64 (FcRI), one of the Fc receptors for IgG, is a membrane glycoprotein that mediates endocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide production. CD64 is also structurally distinct, containing an extracellular Ig-interactive region of three Ig-like domains in contrast to the two domains of the low affinity receptors FcRII and FcRIII. It is normally expressed on the surfaces of monocytes and macrophages.